Preview

The Journal of Almaty Technological University

Advanced search

Changes in the amino acid profile of soft goat milk cheese upon the addition of biologically active components

https://doi.org/10.48184/2304-568X-2026-1-104-113

Abstract

This article examines the effect of propolis addition on the amino acid profile of soft cheese made from goat's milk. Ethanol propolis extract was added to the cheese in concentrations of 0.5%, 1% and 2%. Methods included high performance liquid chromatography for quantification of amino acids, microbiological testing for pathogen inhibition, physico-chemical analysis of product stability, and organoleptic assessment of consumer acceptability. The results show that propolis minimally changes the total amino acid content, but significantly increases the content of free amino acids (for example, proline by 8-15%, lysine by 4-8% and branched chain amino acids by 3-6%) due to improved microbiological stability and controlled proteolysis. Cheese retains a high level of essential amino acids, the ratio of total amino acids is 42-43%, which makes it suitable for dietary use in people with weakened immune systems or in need of high-protein nutrition. Propolis also prolongs the shelf life by reducing the growth of pathogenic microorganisms by 80-95%.

About the Authors

D. T. Tapalov
https://orcid.org/0009-0000-6066-0260
«Almaty Technological University» JSC
Kazakhstan

050012, Almaty, Tole bi str., 100



T. K. Kulazhanov
«Almaty Technological University» JSC
Kazakhstan

050012, Almaty, Tole bi str., 100



A. I. Matibaeva
«Almaty Technological University» JSC
Kazakhstan

050012, Almaty, Tole bi str., 100



B. Sh. Jetpisbayeva
«Almaty Technological University» JSC
Kazakhstan

050012, Almaty, Tole bi str., 100



A. A. Mussayev
«Almaty Technological University» JSC
Kazakhstan

050012, Almaty, Tole bi str., 100



References

1. Park Y.W. Goat milk – chemistry and nutrition // Handbook of Milk of Non-Bovine Mammals. – Wiley, 2017. – P. 42–83. https://doi.org/10.1002/9781119 110316.ch2.2

2. Pedonese F., Verani G., Torracca B., Turchi B., Felicioli A., Nuvoloni R. Effect of an Italian propolis on the growth of Listeria monocytogenes, Staphylococcus aureus and Bacillus cereus in milk and whey cheese // Italian Journal of Food Safety. – 2019. – Vol. 8(4). https://doi.org/10.4081/ijfs.2019.8036

3. Jia R., Lou Y., Zhang F., Liu Y., Wang W., Peng H., Hui Y., Wang B. Amino acid composition and nutritional evaluation of proteins in goat cheeses produced with different starter cultures // Journal of Food and Nutrition Research. – 2022. – Vol. 10(9). – P. 600–607. https://doi.org/10.12691/jfnr-10-9-3

4. Camacho-Bernal G.I., Cruz-Cansino N.S., Ramírez-Moreno E., Delgado-Olivares L., Zafra-Rojas Q.Y., Castañeda-Ovando A., Suárez-Jacobo Á. Addition of bee products in diverse food sources: functional and physicochemical properties // Applied Sciences. – 2021. – Vol. 11(17). – 8156. https://doi.org/10.3390/app11178156

5. Rendueles E., Mauriz E., Sanz-Gómez J., Adanero-Jorge F., García-Fernandez C. Antimicrobial activity of Spanish propolis against Listeria monocytogenes and other Listeria strains // Microorganisms. – 2023. – Vol. 11(6). – 1429. https://doi.org/10.3390/microorganisms11061429

6. Iquiapaza I.Y.C., Aguilar G.J., Zitei-Baptista L.F., Trindade D.M., da Silva Ferreira M.E., Tapia- Blácido D.R. Effect of Brazilian green propolis extracts on the properties of Andean potato starch–chitosan blended films and their use in preserving Parmesan cheese // International Journal of Biological Macro- molecules. – 2025. – Vol. 321. – 146551. https:// doi.org/10.1016/j.ijbiomac.2025.146551

7. Borba K.K.S., Gadelha T.S., Sant’Ana A.M.S., Pacheco M.T.B., Pinto L.S., Madruga M.S., Medeiros A.N., Bessa R.J.B., Alves S.P.A., Magnani M., Pimentel T.C., Queiroga R.C.R.E. Fatty acids, essential amino acids, minerals and proteins profile in whey from goat cheese: impacts of raising system // Small Ruminant Research. – 2022. – Vol. 217. – 106842. https://doi.org/10.1016/j.smallrumres.2022.106842

8. Bankova V. Chemical diversity of propolis and the problem of standardization // Journal of Ethnopharmacology. – 2005. – Vol. 100(1–2). – P. 114– 117. https://doi.org/10.1016/j.jep.2005.05.004

9. Park Y.W., Haenlein G.F.W. (eds.). Handbook of Milk of Non-Bovine Mammals. – Wiley, 2006. https://doi.org/10.1002/9780470999738

10. Matibaeva A.I., Mukhtarkhanova R.B., Dzhepisbaeva B.Sh., Nagin A.V., Zhaksylyk A.Zh. Innovatsionnye metody snizheniya sebestoimosti proizvodstva solenogo syra: novye tekhnologii i podkhody [Innovative methods for reducing the cost of salted cheese production: new technologies and approaches] // Vestnik Almatinskogo tekhnolo- gicheskogo universiteta. – 2025. – No. 148(2). – P. 17– 27. https://doi.org/10.48184/2304-568x-2025-2-17-27 (in Russian)

11. Matibaeva A.I., Mukhtarkhanova R.B., Dzhetpisbaeva B.Sh., Nagin A.V. Sposob posola sychuzhnogo syra [Method for salting rennet cheese]. Patent for utility model No. 11395, 14.11.2025. (in Russian)

12. Tapalov D.T., Zhaksylyk A.Zh. Funktsi- onal'nye i organolepticheskie kharakteristiki myagkikh syrov, obogashchennykh probiotikami [Functional and organoleptic characteristics of soft cheeses enriched with probiotics] // Internauka. – 2025. – No. 16(380). – P. 54–55. (in Russian)

13. Tapalov D.T., Matibaeva A.I., Zhaksylyk A.Zh. Povyshenie pishchevoi tsennosti kislomo- lochnykh produktov za schet ispol'zovaniya meda i produktov pchelovodstva [Increasing the nutritional value of fermented milk products using honey and bee products] // Internauka. – 2025. – No. 16(380). – P. 52– 53. (in Russian)

14. Orazbay A.Zh., Matibaeva A.I., Dzhetpis- baeva B.Sh., Zhaksylyk A. Propolisti ashytılğan süt önimderi tehnologiyasynda qoldanu arqyly antioksidanttyq belsendilikti arttıru [Increasing antioxidant activity through the use of propolis in fermented milk product technology] // Republican scientific-practical conference of young scientists “Gylym. Bilim. Zhastar”. – Almaty: ATU, 2025. – P. 33–35. (in Kazakh)

15. Matibayeva A., Jetpisbayeva B., Zhexenbay N., Izteliyeva R., Kuzembayeva G., Abdiyeva K. Investigation of the effect of technological processing on the quality of goat’s milk cheese // Current Research in Nutrition and Food Science Journal. – 2022. – Vol. 10(1). – P. 213–220. https://doi.org/10.12944/crnfsj.10.1.16


Review

For citations:


Tapalov D.T., Kulazhanov T.K., Matibaeva A.I., Jetpisbayeva B.Sh., Mussayev A.A. Changes in the amino acid profile of soft goat milk cheese upon the addition of biologically active components. The Journal of Almaty Technological University. 2026;151(1):104-113. (In Kazakh) https://doi.org/10.48184/2304-568X-2026-1-104-113

Views: 149

JATS XML


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2304-568X (Print)
ISSN 2710-0839 (Online)